Histone H3 (Tri Methyl K36) Recombinant Antibody

Name: Histone H3 (Tri Methyl K36) Recombinant Antibody
Brand: Bioss
SKU: bsm-60113R
Price: 490.0 USD
Availability: InStock

$Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells per IP, with 5 \u03bcg of bsm-60113R antibody or 5 \u03bcg of normal rabbit IgG(negative control bs-0295P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-promoter, GAPDH-CDS, GAPDH-intron 2, GAPDH-intron 2 and \u03b3-Actin intron 3 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.$

Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells per IP, with 5 \u03bcg of bsm-60113R antibody or 5 \u03bcg of normal rabbit IgG(negative control bs-0295P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-promoter, GAPDH-CDS, GAPDH-intron 2, GAPDH-intron 2 and \u03b3-Actin intron 3 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.

Applications

WB
IHC-F
IF
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60113r
Product Name	Histone H3 (Tri Methyl K36) Recombinant Antibody
Applications	WB IHC-F IF IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Monoclonal
Clone #	B3C9
Isotype	IgG
Concentration	Lot Dependent
Purification	Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P68431
Application Dilution
WB
IHC-F
IF
IHC-P

Recombinant Proteins (4).png__PID:b4703663-ad26-4eba-90ef-48f549942e61

bsm-60113r Copy

Data Sheet