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BCL6 Recombinant Antibody

Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates probed with BCL6 Monoclonal Antibody, Unconjugated (bsm-60223R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates probed with BCL6 Monoclonal Antibody, Unconjugated (bsm-60223R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
The Daudi (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-BCL6 antibody (bsm-60223R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Daudi (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-BCL6 antibody (bsm-60223R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Daudi (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (BCL6) monoclonal Antibody, unconjugated (bsm-60223R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Daudi (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (BCL6) monoclonal Antibody, unconjugated (bsm-60223R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • FCM
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-60223r
Product Name BCL6 Recombinant Antibody
Applications
FCM IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human BCL 6
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location Nucleus
Application Dilution
FCM 1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is BCL6 Recombinant Antibody (bsm-60223R) validated for, and at what dilution?

bsm-60223R is validated for FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BCL6 Recombinant Antibody (bsm-60223R) react with?

Tested reactivity: Human.

How should bsm-60223R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BCL6 Recombinant Antibody (bsm-60223R)?

A synthesized peptide derived from human BCL 6

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