SNAP25 Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-60238m-100uL |
| Product Name | SNAP25 Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human SNAP25 |
| Clonality | Recombinant |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 6616 |
| Swiss Prot | P60880 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | SNAP-25; bA416N4.2; Bdr; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SNAP; SNAP-25B; SNP 25; SNP25; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal Associated Protein 25; Synaptosomal associated protein 25kDa; SNP25_HUMAN. |
| Background | Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | =1:1000-2000 |
| IHC-P | IHC-P=1:400-800 |
| IHC-F | 1:100-500 |
| IF | |
Frequently asked questions
Which applications is SNAP25 Recombinant Antibody (bsm-60238M) validated for, and at what dilution?
bsm-60238M is validated for WB (WB=1:1000-2000); IHC-P (IHC-P=1:400-800); IHC-F (1:100-500); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SNAP25 Recombinant Antibody (bsm-60238M) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bsm-60238M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise SNAP25 Recombinant Antibody (bsm-60238M)?
KLH conjugated synthetic peptide derived from human SNAP25