CD35 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60247r-cy3-100uL |
| Product Name | CD35 Recombinant Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD35 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1378 |
| Swiss Prot | P17927 |
| Subcellular location | Cell membrane |
| Synonyms | C3 binding protein; C3b/C4b receptor; C3BR; C4BR; CD 35; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement component (3b/4b) receptor 1 including Knops blood group system; Complement component receptor 1; Complement receptor 1; Complement receptor type 1; CR 1; CR1; CR1_HUMAN; KN; Knops blood group antigen. |
| Background | This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. |
| Application Dilution | |
| IF(IHC-P) | 1:100-500 |
Frequently asked questions
Which applications is CD35 Recombinant Antibody, Cy3 Conjugated (bsm-60247R-Cy3) validated for, and at what dilution?
bsm-60247R-Cy3 is validated for IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD35 Recombinant Antibody, Cy3 Conjugated (bsm-60247R-Cy3) react with?
Tested reactivity: Human.
How should bsm-60247R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD35 Recombinant Antibody, Cy3 Conjugated (bsm-60247R-Cy3)?
KLH conjugated synthetic peptide derived from human CD35
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-60247R-Cy3)?
Cy3 on bsm-60247R-Cy3: excitation / emission 512,550nm/570,615nm.