IKZF1 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60278r-100uL |
| Product Name | IKZF1 Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IKZF1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 10320 |
| Swiss Prot | Q13422 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | IKAROS family zinc finger 1 (Ikaros); PRO0758; CLL associated antigen KW 6; DNA-binding protein Ikaros; hIk 1; hIk-1; IK1; Ikaros (zinc finger protein); IKAROS; Ikaros family zinc finger protein 1; IKZF1_HUMAN; LYF1; Lymphoid transcription factor LyF-1; zinc finger protein subfamily 1A 1; ZNFN1A1. |
| Background | This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014] |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:200-500 |
| IHC-F | ICC=1:50 |
| IF | IF=1:50-100 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is IKZF1 Recombinant Antibody (bsm-60278R) validated for, and at what dilution?
bsm-60278R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:200-500); IHC-F (ICC=1:50); IF (IF=1:50-100); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IKZF1 Recombinant Antibody (bsm-60278R) react with?
Tested reactivity: Human.
How should bsm-60278R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise IKZF1 Recombinant Antibody (bsm-60278R)?
KLH conjugated synthetic peptide derived from human IKZF1