HBA1 Recombinant Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60287r-fitc-100uL |
| Product Name | HBA1 Recombinant Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HBA1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3039 |
| Swiss Prot | P69905 |
| Subcellular location | Cell membrane |
| Synonyms | Alpha 1 globin; Alpha globin; Alpha one globin; Alpha-globin; HBA_HUMAN; HBA2; Hemoglobin alpha 1; Hemoglobin alpha 1 chain; Hemoglobin alpha 1 globin chain; Hemoglobin alpha 2; Hemoglobin alpha chain; Hemoglobin subunit alpha; MGC126895; MGC12689. |
| Background | he human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is HBA1 Recombinant Antibody, FITC Conjugated (bsm-60287R-FITC) validated for, and at what dilution?
bsm-60287R-FITC is validated for IF(IHC-P)(IF(IHC-P)) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HBA1 Recombinant Antibody, FITC Conjugated (bsm-60287R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-60287R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HBA1 Recombinant Antibody, FITC Conjugated (bsm-60287R-FITC)?
KLH conjugated synthetic peptide derived from human HBA1
What are the excitation and emission wavelengths of the FITC conjugate (bsm-60287R-FITC)?
FITC on bsm-60287R-FITC: excitation / emission 494nm/518nm.