CYP11A1 Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-60352m-100uL |
| Product Name | CYP11A1 Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human CYP11A1 |
| Clonality | Recombinant |
| Isotype | IgG1/Kappa |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1583 |
| Swiss Prot | P05108 |
| Subcellular location | Cytoplasm |
| Synonyms | Cholesterol 20 22 desmolase; Cholesterol desmolase; Cholesterol monooxygenase (side chain cleaving); Cholesterol side chain cleavage enzyme; Cholesterol side chain cleavage enzyme mitochondrial; Cholesterol side-chain cleavage enzyme; CP11A_HUMAN; CYP11A1; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450 11A1 mitochondrial; Cytochrome P450 family 11 subfamily A polypeptide 1; Cytochrome P450 subfamily XIA; Cytochrome P450(scc); Cytochrome P450C11A1; mitochondrial; P450SCC; Steroid 20 22 lyase. |
| Background | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:50-1:100 |
| IHC-F | ICC=1:50 |
| IF | IF=1:100-500 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is CYP11A1 Recombinant Antibody (bsm-60352M) validated for, and at what dilution?
bsm-60352M is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:50-1:100); IHC-F (ICC=1:50); IF (IF=1:100-500); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP11A1 Recombinant Antibody (bsm-60352M) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-60352M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise CYP11A1 Recombinant Antibody (bsm-60352M)?
KLH conjugated synthetic peptide derived from human CYP11A1