ERO1L Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HEK-293, 2: Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 54 kDa\r\nObserved MW: 65 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HEK-293, 2: Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 54 kDa\r\nObserved MW: 65 kDa

Applications

WB
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-60388m
Product Name	ERO1L Monoclonal Antibody
Applications	WB, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	A7B5
Isotype	IgG2B/Kappa
Concentration	1mg/ml
Purification	Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	Q96HE7
Application Dilution
WB	=1:1000-1:5000
ICC/IF	ICC/IF=1:50