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Laminin B1 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Laminin B1 monoclonal antibody, unconjugated (bsm-60601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Laminin B1 monoclonal antibody, unconjugated (bsm-60601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Laminin B1 monoclonal antibody, unconjugated (bsm-60601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Laminin B1 monoclonal antibody, unconjugated (bsm-60601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The A431 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Laminin beta 1 antibody (bsm-60601R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
The A431 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Laminin beta 1 antibody (bsm-60601R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60601r
Product Name Laminin B1 Recombinant Antibody
Applications
WB FCM
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human Laminin beta 1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location Secreted, Cell membrane, Extracellular matrix
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is Laminin B1 Recombinant Antibody (bsm-60601R) validated for, and at what dilution?

bsm-60601R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Laminin B1 Recombinant Antibody (bsm-60601R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-60601R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Laminin B1 Recombinant Antibody (bsm-60601R)?

A synthesized peptide derived from human Laminin beta 1

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