Moesin Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: HeLa, 2: Jurkat, 3: C6 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 75 kDa Observed MW: 75 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: HeLa, 2: Jurkat, 3: C6 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 75 kDa Observed MW: 75 kDa

$Tissue: Mouse liver Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60653R$

Tissue: Mouse liver Section type: Formalin-fixed & Paraffin\r\n-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60653R

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60653r
Product Name	Moesin Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Moesin
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4478
Swiss Prot	P26038
Subcellular location	Cytoplasm
Synonyms	Membrane organizing extension spike protein; Moesin/anaplastic lymphoma kinase fusion protein; MSN; MSN/ALK fusion; MOES_HUMAN.
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:50-1:100
IF	IF=1:50