phospho-TBK1 (Ser172) Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate:(-): HeLa, (+): HeLa+ Okadaic A+ Calyculin A (100 nM, 60min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa$

Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate:(-): HeLa, (+): HeLa+ Okadaic A+ Calyculin A (100 nM, 60min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa

$Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: (-): C6, (+): C6+Calyculin A(100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa$

Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: (-): C6, (+): C6+Calyculin A(100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa

$Hela (H) cells were treated with or without Calyculin A (100nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-TBK1 monoclonal antibody, unconjugated (bsm-60659R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Hela (H) cells were treated with or without Calyculin A (100nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-TBK1 monoclonal antibody, unconjugated (bsm-60659R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60659r
Product Name	phospho-TBK1 (Ser172) Recombinant Antibody
Applications	WB
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human phospho-TBK1 (Ser172)
Modification Site	Ser172
Clonality	Monoclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	29110
Swiss Prot	Q9UHD2
Subcellular location	Cytoplasm, Nucleus
Synonyms	EC 2.7.11.1; FLJ11330; NAK; NF kappa B activating kinase; NF kB activating kinase; NF-kappa-B-activating kinase; Serine/threonine protein kinase TBK 1; Serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK binding kinase 1; TANK-binding kinase 1; TBK 1; TBK1_HUMAN.
Application Dilution
WB	1:300-5000