RUNX3 Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: MCF-7(Negative control), 2: Ramos, 3: Raji, 4: HT-1080 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 46 kDa Observed MW: 44-46 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: MCF-7(Negative control), 2: Ramos, 3: Raji, 4: HT-1080 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 46 kDa Observed MW: 44-46 kDa

Applications

Reactivity

Human

Overview
Catalog #	bsm-60684r
Product Name	RUNX3 Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RUNX3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	864
Swiss Prot	Q13761
Subcellular location	Cytoplasm, Nucleus
Synonyms	RUNX3_HUMAN; Runt-related transcription factor 3; AML2; CBFA3; PEBP2A3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3 (CBF-alpha-3); Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit (PEA2-alpha C; PEBP2-alpha C); SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit;
Application Dilution
WB	1:300-5000