PDHA1 Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: HeLa, 2: Mouse kidney, 3: Rat kidney Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 43 kDa Observed MW: 43 kDa\r\n$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: HeLa, 2: Mouse kidney, 3: Rat kidney Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 43 kDa Observed MW: 43 kDa\r\n

Applications

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60685r
Product Name	PDHA1 Recombinant Antibody
Applications	WB
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PDHA1
Clonality	Monoclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	5160
Swiss Prot	P08559
Subcellular location	Cytoplasm
Synonyms	Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; ODPA_HUMAN; EC:1.2.4.1; PDHE1-A type I; pyruvate dehydrogenase E1 subunit alpha 1; PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; PDH-E1_;
Application Dilution
WB	1:300-5000