Alpha Tubulin (Acetyl-Lys40) Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Right temporal lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Right temporal lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Hela (H) cells were treated with or without Trichostatin A (500 ng\/ml ) for 4 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Alpha Tubulin (Acetyl-Lys40) monoclonal antibody, unconjugated (bsm-60706R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Hela (H) cells were treated with or without Trichostatin A (500 ng\/ml ) for 4 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Alpha Tubulin (Acetyl-Lys40) monoclonal antibody, unconjugated (bsm-60706R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60706r
Product Name	Alpha Tubulin (Acetyl-Lys40) Recombinant Antibody
Applications	WB IHC-P IHC-F IF ICC/IF
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Alpha Tubulin (Acetyl-Lys40)
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7277
Swiss Prot	P68366
Subcellular location	Cytoplasm
Synonyms	Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; TBA1A_HUMAN; TBA1B_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A; _ tubulin.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC/IF	1:50-100