Ki67 polyclonal antibody

$Cell line: HeLa\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour\r\nat room temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nCounter stain: Tubulin (Red)\r\nComment: Color green is the positive signal\r\nfor bsm-60738R$

Cell line: HeLa\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour\r\nat room temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nCounter stain: Tubulin (Red)\r\nComment: Color green is the positive signal\r\nfor bsm-60738R

$Tissue: Rat colon\r\nSection type: Formalin fixed & Paraffin -\r\nembedded section\r\nRetrieval method: High temperature and high\r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at\r\nroom temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal\r\nfor bsm-60738R$

Tissue: Rat colon\r\nSection type: Formalin fixed & Paraffin -\r\nembedded section\r\nRetrieval method: High temperature and high\r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at\r\nroom temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal\r\nfor bsm-60738R

$Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -\r\nembedded section\r\nRetrieval method: High temperature and high\r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at\r\nroom temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal\r\nfor bsm-60738R$

Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -\r\nembedded section\r\nRetrieval method: High temperature and high\r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at\r\nroom temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal\r\nfor bsm-60738R

$Tissue: mouse spleen\r\nSection type: Formalin fixed & Paraffin -\r\nembedded section\r\nRetrieval method: High temperature and high\r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at\r\nroom temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal\r\nfor bsm-60738R$

Tissue: mouse spleen\r\nSection type: Formalin fixed & Paraffin -\r\nembedded section\r\nRetrieval method: High temperature and high\r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at\r\nroom temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal\r\nfor bsm-60738R

Applications

IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60738R
Product Name	Ki67 polyclonal antibody
Applications	IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Ki67
Clonality	Monoclonal
Clone #	6B9
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4288
Swiss Prot	P46013
Subcellular location	Nucleus
Synonyms	Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.
Background	Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Application Dilution
IHC-P	1:500-1000
IF(ICC)	1:50-100

Applications

Reactivity

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