Granzyme B Monoclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60779r-cy5-100uL |
| Product Name | Granzyme B Monoclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GZMB |
| Clonality | Monoclonal |
| Clone # | H12A5 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3002 |
| Swiss Prot | P10144 |
| Subcellular location | Secreted, Extracellular domain |
| Synonyms | GranzymeB; Granzyme-B; C11; Cathepsin G-like 1; CTLA-1; Cathespin G Like 1; CCPI; CCP-1; CGL 1; CGL1; CSP B; CSPB; CTLA 1;CTLA1; CTSGL 1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T Lymphocyte Associated Serine Esterase 1; Cytotoxic T lymphocyte proteinase 2; Fragmentin 2; Fragmentin2; Granzyme 2; Granzyme2; GRB; GZMB; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; GRAB_HUMAN; Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1); Granzyme B; Granzyme-2; GranzymeB; HLP; T cell serine protease 1 3E; T cell serine protease 1-3E; T-cell serine protease 1-3E; HLP; Human lymphocyte protein; Lymphocyte protease; Protease Serine B; SECT; AA 18-29; T cell serine protease 1. |
| Background | This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Granzyme B Monoclonal Antibody, Cy5 Conjugated (bsm-60779R-Cy5) validated for, and at what dilution?
bsm-60779R-Cy5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Granzyme B Monoclonal Antibody, Cy5 Conjugated (bsm-60779R-Cy5) react with?
Tested reactivity: Human.
How should bsm-60779R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Granzyme B Monoclonal Antibody, Cy5 Conjugated (bsm-60779R-Cy5)?
KLH conjugated synthetic peptide derived from human GZMB
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-60779R-Cy5)?
Cy5 on bsm-60779R-Cy5: excitation / emission 625,650nm/670nm.