CD74 Monoclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60795r-pe-cy55-100ul |
| Product Name | CD74 Monoclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD74 |
| Clonality | Monoclonal |
| Clone # | E9C4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 972 |
| Swiss Prot | P04233 |
| Subcellular location | Cell membrane |
| Synonyms | CD 74; CD74 antigen; CD74 molecule; CLIP; DHLAG; Gamma chain of class II antigens; HG2A_HUMAN; HLA class II histocompatibility antigen gamma chain; HLA DR antigens associated invariant chain; HLA DR gamma; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLADG; HLADR antigens associated invariant chain; Ia antigen associated invariant chain; Ia antigen-associated invariant chain; Ia associated invariant chain; Ia gamma; Ii; Invariant polypeptide of major histocompatibility complex class II antigen associated; la-gamma; Major histocompatibility complex class II invariant chain; MHC HLA DR gamma chain; MHC HLA-DR gamma chain; p33; p35; Protein 41. |
| Background | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-200 |
Frequently asked questions
Which applications is CD74 Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-60795R-PE-CY5-5) validated for, and at what dilution?
BSM-60795R-PE-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD74 Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-60795R-PE-CY5-5) react with?
Tested reactivity: Human.
How should BSM-60795R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD74 Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-60795R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human CD74
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-60795R-PE-CY5-5)?
PE-Cy5.5 on BSM-60795R-PE-CY5-5: excitation / emission 488nm/694nm.