Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

ACTH Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Human pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACTH Monoclonal Antibody, Unconjugated(bsm-60798R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACTH Monoclonal Antibody, Unconjugated(bsm-60798R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with ACTH monoclonal antibody, unconjugated (bsm-60798R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with ACTH monoclonal antibody, unconjugated (bsm-60798R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTH Monoclonal Antibody, Unconjugated (bsm-60798R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTH Monoclonal Antibody, Unconjugated (bsm-60798R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Mouse
Overview
Catalog # bsm-60798r
Product Name ACTH Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Human, Mouse, Rat
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ACTH
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer PBS, Glycerol, BSA.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location Nucleus
Synonyms Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; alpha Melanocyte Stimulating Hormone; beta Lipotropin; beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin beta; Lipotropin gamma; LPH; Melanotropin alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin; COLI_HUMAN.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:50-200
IHC-F IHC-F1:50-200
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is ACTH Recombinant Antibody (bsm-60798R) validated for, and at what dilution?

bsm-60798R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ACTH Recombinant Antibody (bsm-60798R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.

How should bsm-60798R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: PBS, Glycerol, BSA.

What immunogen was used to raise ACTH Recombinant Antibody (bsm-60798R)?

KLH conjugated synthetic peptide derived from human ACTH

Search our catalog of hundreds of thousands of products