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P53 Recombinant Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-60806r-bf647-100uL
Product Name P53 Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source A synthesized peptide derived from human p53
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7157
Swiss Prot P04637
Subcellular location Cytoplasm, Nucleus
Synonyms P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53;
Application Dilution
WB 1:2000-20000
FCM FCM1ug/Test
IF(IHC-P) IFIHC-P1:200-1000
IF(ICC) IFICC1:100-200

Frequently asked questions

Which applications is P53 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-60806R-BF647) validated for, and at what dilution?

bsm-60806R-BF647 is validated for WB(WB) (1:2000-20000); FCM(FCM) (1ug/Test); IF(IHC-P)(IF(IHC-P)) (1:200-1000); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does P53 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-60806R-BF647) react with?

Tested reactivity: Human.

How should bsm-60806R-BF647 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise P53 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-60806R-BF647)?

A synthesized peptide derived from human p53

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-60806R-BF647)?

AbBy Fluor-647 on bsm-60806R-BF647: excitation / emission 650nm/665nm.

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