PAP Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded (Human prostate tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PAP) Monoclonal Antibody, Unconjugated (bsm-60820R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human prostate tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PAP) Monoclonal Antibody, Unconjugated (bsm-60820R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-60820r
Product Name	PAP Recombinant Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Prostatic Acid Phosphatase
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55
Swiss Prot	P15309
Subcellular location	Secreted, Cell membrane
Synonyms	Acid phosphatase prostate; ACP 3; ACP3; ACPP; Ecto-5'-nucleotidase; Prostatic Acid Phosphatase ; PPAP_HUMAN; Prostatic acid phosphatase; Thiamine monophosphatase; TMPase; 5'-NT; 5'-nucleotidase.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF