CD34 Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60829r-apc-cy5 |
| Product Name | CD34 Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD34 |
| Clonality | Monoclonal |
| Clone # | 4G1 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 947 |
| Swiss Prot | P28906 |
| Subcellular location | Cell membrane |
| Synonyms | CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN. |
| Background | The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CD34 Monoclonal Antibody, APC-Cy5 Conjugated (bsm-60829R-APC-Cy5) validated for, and at what dilution?
bsm-60829R-APC-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD34 Monoclonal Antibody, APC-Cy5 Conjugated (bsm-60829R-APC-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-60829R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD34 Monoclonal Antibody, APC-Cy5 Conjugated (bsm-60829R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human CD34
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-60829R-APC-Cy5)?
APC-Cy5 on bsm-60829R-APC-Cy5: excitation / emission 650nm/670nm.