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L-Lactyl Lysine Recombinant Antibody

4% Paraformaldehyde-fixed HeLa (treated with sodium lactate (100mM, 24hr)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (L-Lactyl Lysine) monoclonal Antibody, unconjugated (bsm-60863R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (treated with sodium lactate (100mM, 24hr)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (L-Lactyl Lysine) monoclonal Antibody, unconjugated (bsm-60863R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HeLa (treated with sodium lactate (100mM, 24hr)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-L-Lactyl Lysine antibody (bsm-60863R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (treated with sodium lactate (100mM, 24hr)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-L-Lactyl Lysine antibody (bsm-60863R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IHC-P
  • IHC-F
  • IP
  • IF
  • ChIP
  • ICC/IF

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bsm-60863r
Product Name L-Lactyl Lysine Recombinant Antibody
Applications
WB FCM IHC-P IHC-F IP IF CHIP ICC/IF
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source L-Lactyl Lysine-KLH
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer PBS, Glycerol, BSA
Storage Condition Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Background

Lactyl lysine is derivative of the amino acid residue lysine where the sidechain ammonium group undergoes lactylation.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IP 1-2ug
IF
ChIP
ICC/IF 1:50-100

Frequently asked questions

Which applications is L-Lactyl Lysine Recombinant Antibody (bsm-60863R) validated for, and at what dilution?

bsm-60863R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IP (1-2ug); IF; ChIP; ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does L-Lactyl Lysine Recombinant Antibody (bsm-60863R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bsm-60863R be stored?

Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: PBS, Glycerol, BSA

What immunogen was used to raise L-Lactyl Lysine Recombinant Antibody (bsm-60863R)?

L-Lactyl Lysine-KLH

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