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Glutaminase Monoclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60888r-pe-100uL
Product Name Glutaminase Monoclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GLS1
Clonality Monoclonal
Clone # No
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2744
Swiss Prot O94925
Subcellular location Cytoplasm
Synonyms mitochondrial; AAD20; GLS; GLS1; GLSK_HUMAN; Glutaminase kidney isoform; K-glutaminase; KIAA0838; L-glutamine amidohydrolase; DKFZp686O15119; FLJ10358; GAC; GAM; Glutaminase C; Glutaminase phosphate activated.
Background Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Application Dilution
WB 1:500-2000
FCM FCM1ug/Test
IF(IHC-P) IFIHC-P1:200-500
IF(ICC) IFICC1:100-200

Frequently asked questions

Which applications is Glutaminase Monoclonal Antibody, PE Conjugated (bsm-60888R-PE) validated for, and at what dilution?

bsm-60888R-PE is validated for WB(WB) (1:500-2000); FCM(FCM) (1ug/Test); IF(IHC-P)(IF(IHC-P)) (1:200-500); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Glutaminase Monoclonal Antibody, PE Conjugated (bsm-60888R-PE) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-60888R-PE be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Glutaminase Monoclonal Antibody, PE Conjugated (bsm-60888R-PE)?

KLH conjugated synthetic peptide derived from human GLS1

What are the excitation and emission wavelengths of the PE conjugate (bsm-60888R-PE)?

PE on bsm-60888R-PE: excitation / emission 496,564nm/578nm.