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PKC zeta Recombinant Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-60921r-bf680
Product Name PKC zeta Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
WB
Reactivity Human, Mouse
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PKC zeta
Clonality Recombinant
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5590
Swiss Prot Q05513
Subcellular location Cytoplasm
Synonyms AI098070; aPKCzeta; C80388; EC 2.7.11.13; KPCZ_HUMAN; nPKC zeta; nPKC-zeta; OTTHUMP00000001368; OTTHUMP00000044160; PKC 2; PKC ZETA; PKC2; Pkcz; PKCZETA; PKM-zeta, included; PRKCZ; Protein kinase C zeta; Protein kinase C zeta form; Protein kinase C zeta type; r14-3-3; R74924; zetaPKC; PKC-ZETA; PKC2; nPKC-zeta.
Background Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:500-1000

Frequently asked questions

Which applications is PKC zeta Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-60921R-BF680) validated for, and at what dilution?

bsm-60921R-BF680 is validated for WB(WB) (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PKC zeta Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-60921R-BF680) react with?

Tested reactivity: Human, Mouse.

How should bsm-60921R-BF680 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PKC zeta Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-60921R-BF680)?

KLH conjugated synthetic peptide derived from human PKC zeta

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-60921R-BF680)?

AbBy Fluor-680 on bsm-60921R-BF680: excitation / emission 679nm/702nm.