WT1 Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60926r-bf555 |
| Product Name | WT1 Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human WT1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7490 |
| Swiss Prot | P19544 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | WT1_HUMAN; Wilms tumor protein; WT33; WT1 transcription factor; GUD; AWT1; WAGR; WT-1; WT33; NPHS4; WIT-2; |
| Background | Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:500-1000 |
Frequently asked questions
Which applications is WT1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-60926R-BF555) validated for, and at what dilution?
bsm-60926R-BF555 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does WT1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-60926R-BF555) react with?
Tested reactivity: Human, Mouse.
How should bsm-60926R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise WT1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-60926R-BF555)?
KLH conjugated synthetic peptide derived from human WT1
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-60926R-BF555)?
AbBy Fluor-555 on bsm-60926R-BF555: excitation / emission 553nm/568nm.