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SNAI1 Recombinant Antibody

The A549 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SNAI1 antibody (bsm-60947R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The A549 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SNAI1 antibody (bsm-60947R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with SNAI1 monoclonal antibody, unconjugated (bsm-60947R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with SNAI1 monoclonal antibody, unconjugated (bsm-60947R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed A549 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SNAI1) monoclonal Antibody, unconjugated (bsm-60947R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed A549 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SNAI1) monoclonal Antibody, unconjugated (bsm-60947R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-60947r-100uL
Product Name SNAI1 Recombinant Antibody
Applications
WB FCM
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human SNAI1 protein
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6615
Subcellular location Cytoplasm, Nucleus
Synonyms SNAI1_HUMAN; Zinc finger protein SNAI1; SNAI1; SNAH; SNAI 1; SNA; SNAIL1; dJ710H13.1; Protein snail homolog 1(Protein sna); Protein snail homolog 1; Protein sna;
Application Dilution
WB 1:500-2000
FCM FCM1ug/Test

Frequently asked questions

Which applications is SNAI1 Recombinant Antibody (bsm-60947R) validated for, and at what dilution?

bsm-60947R is validated for WB(WB) (1:500-2000); FCM(FCM) (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SNAI1 Recombinant Antibody (bsm-60947R) react with?

Tested reactivity: Human.

How should bsm-60947R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SNAI1 Recombinant Antibody (bsm-60947R)?

Recombinant human SNAI1 protein

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