Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

RNA Polymerase II CTD Repeat YSPTSPS (S2) Recombinant Antibody

Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells (treated with 1.5 mM EGS for 30mins and then formaldehyde for 10mins) per IP, with 6 \u03bcg of bsm-60975R antibody or 6 \u03bcg of normal rabbit IgG(negative control bs-0295P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, GAPDH promoter ,GAPDH intron 2, GAPDH intron 2, \u03b3-Actin intron 3 and \u03b2-actin 3' UTR regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.
Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells (treated with 1.5 mM EGS for 30mins and then formaldehyde for 10mins) per IP, with 6 \u03bcg of bsm-60975R antibody or 6 \u03bcg of normal rabbit IgG(negative control bs-0295P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, GAPDH promoter ,GAPDH intron 2, GAPDH intron 2, \u03b3-Actin intron 3 and \u03b2-actin 3' UTR regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • ChIP
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60975r-100ul
Product Name RNA Polymerase II CTD Repeat YSPTSPS (S2) Recombinant Antibody
Applications
WB IHC-F CHIP IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II CTD around the phosphorylation site of Ser2
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5430
Swiss Prot P24928
Subcellular location Nucleus
Synonyms NEDHIB; POLR2; POLRA; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1; 220kDa; Rpo2-1; RPB1_HUMAN; POLR2A; RNA polymerase II subunit B1; 3'-5' exoribonuclease; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; 2.7.7.6; RPB1_MOUSE; Rpii215; RNA polymerase II subunit A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; POLR2A | RNA polymerase II CTD repeat YSPTSPS (phospho-S2); p-RNA polymerase II CTD repeat YSPTSPS; phospho-RNA polymerase II CTD repeat YSPTSPS; p-POLR2A; POLR2A | RNA polymerase II CTD repeat YSPTSPS (phospho-Ser2)
Application Dilution
WB 1:500-2000
IHC-F 1:50-200
ChIP 6μg/IP
IHC-P 1:50-200

Frequently asked questions

Which applications is RNA Polymerase II CTD Repeat YSPTSPS (S2) Recombinant Antibody (bsm-60975R) validated for, and at what dilution?

bsm-60975R is validated for WB (1:500-2000); IHC-F (1:50-200); ChIP (6μg/IP); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RNA Polymerase II CTD Repeat YSPTSPS (S2) Recombinant Antibody (bsm-60975R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-60975R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RNA Polymerase II CTD Repeat YSPTSPS (S2) Recombinant Antibody (bsm-60975R)?

KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II CTD around the phosphorylation site of Ser2

Search our catalog of hundreds of thousands of products