RalA Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61044r-bf555-100uL |
| Product Name | RalA Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RalA |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane, Extracellular matrix |
| Synonyms | Ral a; Ral A protein; RAL; RALA; RALA_HUMAN; RAS like protein A; Ras related protein RalA; Ras-related protein Ral-A. |
| Background | Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is RalA Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61044R-BF555) validated for, and at what dilution?
bsm-61044R-BF555 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RalA Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61044R-BF555) react with?
Tested reactivity: Human.
How should bsm-61044R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RalA Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61044R-BF555)?
KLH conjugated synthetic peptide derived from human RalA
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-61044R-BF555)?
AbBy Fluor-555 on bsm-61044R-BF555: excitation / emission 553nm/568nm.