Frizzled 8 Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61049r-bf350-100uL |
| Product Name | Frizzled 8 Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Frizzled 8 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8325 |
| Swiss Prot | Q9H461 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Frizzled 8 seven transmembrane spanning receptor; frizzled 8, seven transmembrane spanning receptor; Frizzled family receptor 8; frizzled homolog 8 (Drosophila); Frizzled homolog 8; Frizzled-8; FZ 8; Fz-8; FZ8; FZD 8; FZD8; FZD8_HUMAN; hFZ 8; hFZ8; HGNC4046; Homolog of Drosophila Frizzled 8. |
| Background | Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1 |
| Application Dilution | |
| IF(IHC-P) | 1:100-500 |
Frequently asked questions
Which applications is Frizzled 8 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61049R-BF350) validated for, and at what dilution?
bsm-61049R-BF350 is validated for IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Frizzled 8 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61049R-BF350) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61049R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Frizzled 8 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61049R-BF350)?
KLH conjugated synthetic peptide derived from human Frizzled 8
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-61049R-BF350)?
AbBy Fluor-350 on bsm-61049R-BF350: excitation / emission 346nm/442nm.