LGR5/GPR49 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61052r-cy5-100uL |
| Product Name | LGR5/GPR49 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GPR49 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8549 |
| Swiss Prot | O75473 |
| Subcellular location | Cell membrane |
| Synonyms | G protein coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; GPCR GPR49; G protein coupled receptor 67; FEX; g protein-coupled receptor fex; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; Leucine rich repeat containing G protein coupled receptor 5; HG 38; HG38; LGR5_HUMAN; MGC117008; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; Orphan G protein coupled receptor HG38. |
| Background | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is LGR5/GPR49 Recombinant Antibody, Cy5 Conjugated (bsm-61052R-Cy5) validated for, and at what dilution?
bsm-61052R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LGR5/GPR49 Recombinant Antibody, Cy5 Conjugated (bsm-61052R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61052R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LGR5/GPR49 Recombinant Antibody, Cy5 Conjugated (bsm-61052R-Cy5)?
KLH conjugated synthetic peptide derived from human GPR49
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61052R-Cy5)?
Cy5 on bsm-61052R-Cy5: excitation / emission 625,650nm/670nm.