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AMPK alpha (Thr172) Monoclonal Antibody, APC Conjugated

Applications

  • WB

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-61057r-apc-100uL
Product Name AMPK alpha (Thr172) Monoclonal Antibody, APC Conjugated
Applications
WB
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation APC
Host Rabbit
Source A synthesized peptide derived from human AMPK alpha 1 around the phosphorylation site of T183
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm, Nucleus
Synonyms PRKAA1 | AMPK alpha-1 (p-T172); p-AMPK alpha-1; phospho-AMPK alpha-1; AMPKalpha1; C130083N04Rik; AAPK1_MOUSE; Prkaa1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase (ACACA kinase); Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase); Tau-protein kinase PRKAA1; 2.7.11.1; AAPK1_RAT; Ampk1; AMPK; AMPK alpha 1; AMPKa1;
Application Dilution
WB =1:500-2000

Frequently asked questions

Which applications is AMPK alpha (Thr172) Monoclonal Antibody, APC Conjugated (bsm-61057R-APC) validated for, and at what dilution?

bsm-61057R-APC is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AMPK alpha (Thr172) Monoclonal Antibody, APC Conjugated (bsm-61057R-APC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-61057R-APC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AMPK alpha (Thr172) Monoclonal Antibody, APC Conjugated (bsm-61057R-APC)?

A synthesized peptide derived from human AMPK alpha 1 around the phosphorylation site of T183

What are the excitation and emission wavelengths of the APC conjugate (bsm-61057R-APC)?

APC on bsm-61057R-APC: excitation / emission 650nm/660nm.