Bcl-2 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61074r-rbitc-100uL |
| Product Name | Bcl-2 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Bcl-2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN. |
| Background | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is Bcl-2 Recombinant Antibody, RBITC Conjugated (bsm-61074R-RBITC) validated for, and at what dilution?
bsm-61074R-RBITC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bcl-2 Recombinant Antibody, RBITC Conjugated (bsm-61074R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61074R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bcl-2 Recombinant Antibody, RBITC Conjugated (bsm-61074R-RBITC)?
KLH conjugated synthetic peptide derived from human Bcl-2
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61074R-RBITC)?
RBITC on bsm-61074R-RBITC: excitation / emission 570nm/595nm.