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Galectin 3 Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-61087r-cy3-100uL
Product Name Galectin 3 Recombinant Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Galectin 3
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3958
Swiss Prot P17931
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms Galectin-3; 35 kDa lectin; Carbohydrate binding protein 35; CBP 35; CBP35; GAL 3; GAL3; GAL-3; Galactose specific lectin 3; Galactose-specific lectin 3; Galactoside binding protein; Galactoside-binding protein; GALBP; Galectin3 internal gene,included; GALIG; GBP; IgE binding protein; IgE-binding protein; L 31; L 34; L-34 galactoside-binding lectin; L31; Laminin binding protein; Laminin-binding protein; Lectin galactose binding soluble 3; Lectin galactoside binding soluble 3; Lectin L 29; Lectin L-29; Lectin, galactose binding, soluble 3; LGALS 3; LGALS2; LGALS3; LGALS3 protein; MAC 2 antigen; Mac-2; MAC2; Macrophage galactose-specific lectin; MGC105387; LEG3_HUMAN.
Background Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
Application Dilution
WB 1:1000-5000
IF(IHC-P) IFIHC-P1:200-800

Frequently asked questions

Which applications is Galectin 3 Recombinant Antibody, Cy3 Conjugated (bsm-61087R-Cy3) validated for, and at what dilution?

bsm-61087R-Cy3 is validated for WB(WB) (1:1000-5000); IF(IHC-P)(IF(IHC-P)) (1:200-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Galectin 3 Recombinant Antibody, Cy3 Conjugated (bsm-61087R-Cy3) react with?

Tested reactivity: Human.

How should bsm-61087R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Galectin 3 Recombinant Antibody, Cy3 Conjugated (bsm-61087R-Cy3)?

KLH conjugated synthetic peptide derived from human Galectin 3

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61087R-Cy3)?

Cy3 on bsm-61087R-Cy3: excitation / emission 512,550nm/570,615nm.

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