RNF20 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61100r-rbitc-100uL |
| Product Name | RNF20 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF20 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56254 |
| Swiss Prot | Q5VTR2 |
| Subcellular location | Nucleus |
| Synonyms | BRE1-A; hBRE1; RING finger protein 20; E3 ubiquitin-protein ligase BRE1A; RNF20. |
| Background | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is RNF20 Recombinant Antibody, RBITC Conjugated (bsm-61100R-RBITC) validated for, and at what dilution?
bsm-61100R-RBITC is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF20 Recombinant Antibody, RBITC Conjugated (bsm-61100R-RBITC) react with?
Tested reactivity: Human, Mouse.
How should bsm-61100R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF20 Recombinant Antibody, RBITC Conjugated (bsm-61100R-RBITC)?
KLH conjugated synthetic peptide derived from human RNF20
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61100R-RBITC)?
RBITC on bsm-61100R-RBITC: excitation / emission 570nm/595nm.