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CD97 Recombinant Antibody

4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (CD97) monoclonal Antibody, unconjugated (bsm-61103R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (CD97) monoclonal Antibody, unconjugated (bsm-61103R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Jurkat (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD97 antibody (bsm-61103R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488(bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD97 antibody (bsm-61103R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488(bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IF(ICC)
  • IP

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-61103r
Product Name CD97 Recombinant Antibody
Applications
WB FCM IF(ICC) IP
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ADGRE5
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 976
Swiss Prot P48960
Subcellular location Extracellular, Secreted, Cell membrane
Synonyms ADGRE5; Adhesion G protein-coupled receptor E5; CD97 antigen; Leukocyte antigen CD97; Seven span transmembrane protein; TM7LN1.
Background Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200
IP IP1:20-50

Frequently asked questions

Which applications is CD97 Recombinant Antibody (bsm-61103R) validated for, and at what dilution?

bsm-61103R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD97 Recombinant Antibody (bsm-61103R) react with?

Tested reactivity: Human, Mouse.

How should bsm-61103R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD97 Recombinant Antibody (bsm-61103R)?

KLH conjugated synthetic peptide derived from human ADGRE5

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