Phospho-CREB-1 (Ser133) Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61105r-apc-100uL |
| Product Name | Phospho-CREB-1 (Ser133) Recombinant Antibody, APC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CREB1 around the phosphorylation site of S133 |
| Modification Site | Ser131 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1385 |
| Swiss Prot | P16220 |
| Subcellular location | Nucleus |
| Synonyms | cAMP responsive element binding protein 1; CREB1; CREB-1. |
| Background | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Phospho-CREB-1 (Ser133) Recombinant Antibody, APC Conjugated (bsm-61105R-APC) validated for, and at what dilution?
bsm-61105R-APC is validated for FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CREB-1 (Ser133) Recombinant Antibody, APC Conjugated (bsm-61105R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61105R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CREB-1 (Ser133) Recombinant Antibody, APC Conjugated (bsm-61105R-APC)?
KLH conjugated synthetic peptide derived from human CREB1 around the phosphorylation site of S133
What are the excitation and emission wavelengths of the APC conjugate (bsm-61105R-APC)?
APC on bsm-61105R-APC: excitation / emission 650nm/660nm.