Shh Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61111r-cy3-100uL |
| Product Name | Shh Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sonic hedgehog protein |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6469 |
| Swiss Prot | Q15465 |
| Synonyms | TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5; Sonic Hedgehog. |
| Background | The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity).Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity).Both activities occur in the reticulum endoplasmic (By similarity). |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Shh Recombinant Antibody, Cy3 Conjugated (bsm-61111R-Cy3) validated for, and at what dilution?
bsm-61111R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Shh Recombinant Antibody, Cy3 Conjugated (bsm-61111R-Cy3) react with?
Tested reactivity: Human.
How should bsm-61111R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Shh Recombinant Antibody, Cy3 Conjugated (bsm-61111R-Cy3)?
KLH conjugated synthetic peptide derived from human Sonic hedgehog protein
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61111R-Cy3)?
Cy3 on bsm-61111R-Cy3: excitation / emission 512,550nm/570,615nm.