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GM130 Recombinant Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Monkey
Overview
Catalog # bsm-61120r-apc-100uL
Product Name GM130 Recombinant Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Monkey
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Golgin subfamily A member 2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2801
Swiss Prot Q08379
Subcellular location Cytoplasm, Cell membrane
Synonyms GM130; Gm130 autoantigen; GOLGA 2; Golga2; Golgi autoantigen; Golgin 95.
Background Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is GM130 Recombinant Antibody, APC Conjugated (bsm-61120R-APC) validated for, and at what dilution?

bsm-61120R-APC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GM130 Recombinant Antibody, APC Conjugated (bsm-61120R-APC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Monkey.

How should bsm-61120R-APC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GM130 Recombinant Antibody, APC Conjugated (bsm-61120R-APC)?

KLH conjugated synthetic peptide derived from human Golgin subfamily A member 2

What are the excitation and emission wavelengths of the APC conjugate (bsm-61120R-APC)?

APC on bsm-61120R-APC: excitation / emission 650nm/660nm.