GM130 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61120r-bf488-100uL |
| Product Name | GM130 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Monkey |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Golgin subfamily A member 2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2801 |
| Swiss Prot | Q08379 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | GM130; Gm130 autoantigen; GOLGA 2; Golga2; Golgi autoantigen; Golgin 95. |
| Background | Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is GM130 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61120R-BF488) validated for, and at what dilution?
bsm-61120R-BF488 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GM130 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61120R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Monkey.
How should bsm-61120R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GM130 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61120R-BF488)?
KLH conjugated synthetic peptide derived from human Golgin subfamily A member 2
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61120R-BF488)?
AbBy Fluor-488 on bsm-61120R-BF488: excitation / emission 499nm/519nm.