API2/BIRC3 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61124r-cy3-100uL |
| Product Name | API2/BIRC3 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BIRC3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 330 |
| Swiss Prot | Q13489 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | AIP1; API2; BIRC3; CIAP2; HAIP1; IAP homolog C ;RNF49; MIHC; MALT2; Apoptosis inhibitor 2;IAP2. |
| Background | Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-100 |
Frequently asked questions
Which applications is API2/BIRC3 Recombinant Antibody, Cy3 Conjugated (bsm-61124R-Cy3) validated for, and at what dilution?
bsm-61124R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does API2/BIRC3 Recombinant Antibody, Cy3 Conjugated (bsm-61124R-Cy3) react with?
Tested reactivity: Human.
How should bsm-61124R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise API2/BIRC3 Recombinant Antibody, Cy3 Conjugated (bsm-61124R-Cy3)?
KLH conjugated synthetic peptide derived from human BIRC3
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61124R-Cy3)?
Cy3 on bsm-61124R-Cy3: excitation / emission 512,550nm/570,615nm.