TRAM2 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61131r-rbitc |
| Product Name | TRAM2 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TRAM2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9697 |
| Swiss Prot | Q15035 |
| Subcellular location | Cell membrane |
| Synonyms | KIAA0057;TRAM like protein;Tram2;Translocating chain associating membrane protein 2;Translocation associated membrane protein 2. |
| Background | Necessary for collagen type I synthesis. May couple the activity of the ER Ca2+ pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca2+ concentration at the site of collagen synthesis, and a high Ca2+ concentration may be necessary for the function of molecular chaperones involved in collagen folding. Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is TRAM2 Recombinant Antibody, RBITC Conjugated (bsm-61131R-RBITC) validated for, and at what dilution?
bsm-61131R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TRAM2 Recombinant Antibody, RBITC Conjugated (bsm-61131R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61131R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRAM2 Recombinant Antibody, RBITC Conjugated (bsm-61131R-RBITC)?
KLH conjugated synthetic peptide derived from human TRAM2
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61131R-RBITC)?
RBITC on bsm-61131R-RBITC: excitation / emission 570nm/595nm.
