CD146 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61136r-cy3-100uL |
| Product Name | CD146 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MCAM |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4162 |
| Swiss Prot | P43121 |
| Subcellular location | Cell membrane |
| Synonyms | A32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein P1H12; Gicerin; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32. |
| Background | CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is CD146 Recombinant Antibody, Cy3 Conjugated (bsm-61136R-Cy3) validated for, and at what dilution?
bsm-61136R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD146 Recombinant Antibody, Cy3 Conjugated (bsm-61136R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61136R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD146 Recombinant Antibody, Cy3 Conjugated (bsm-61136R-Cy3)?
KLH conjugated synthetic peptide derived from human MCAM
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61136R-Cy3)?
Cy3 on bsm-61136R-Cy3: excitation / emission 512,550nm/570,615nm.