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HSPA12A Recombinant Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bsm-61137r-rbitc-100uL
Product Name HSPA12A Recombinant Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hsp70 12A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 259217
Swiss Prot O43301
Subcellular location Cytoplasm
Synonyms FLJ13874 antibody;Heat shock 70 kDa protein 12A antibody;heat shock 70kD protein 12A antibody;heat shock 70kDa protein 12A antibody;HS12A_HUMAN antibody;Hspa12a antibody;KIAA0417 antibody.
Background Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is HSPA12A Recombinant Antibody, RBITC Conjugated (bsm-61137R-RBITC) validated for, and at what dilution?

bsm-61137R-RBITC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HSPA12A Recombinant Antibody, RBITC Conjugated (bsm-61137R-RBITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bsm-61137R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HSPA12A Recombinant Antibody, RBITC Conjugated (bsm-61137R-RBITC)?

KLH conjugated synthetic peptide derived from human Hsp70 12A

What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61137R-RBITC)?

RBITC on bsm-61137R-RBITC: excitation / emission 570nm/595nm.