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p57 Kip2/Cdkn1c Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2\/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(ICC)
  • IP

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61145r-TR
Product Name p57 Kip2/Cdkn1c Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P) IF(ICC) IP
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human p57 Kip2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1028
Swiss Prot P49918
Subcellular location Nucleus
Synonyms BWCR; BWS; KIP2; WBS; p57; p57 Kip2; WBS ; CDKN1C; Cyclin dependent kinase inhibitor 1C.
Background Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
Application Dilution
WB 1:1000-2000
IHC-P IHC-P1:100-200
IHC-F IHC-F1:100-200
IF(IHC-P) IFIHC-P1:100-200
IF(ICC) IFICC1:50-200
IP IP1:20-50

Frequently asked questions

Which applications is p57 Kip2/Cdkn1c Recombinant Antibody (bsm-61145R) validated for, and at what dilution?

bsm-61145R is validated for WB(WB) (1:1000-2000); IHC-P(IHC-P) (1:100-200); IHC-F(IHC-F) (1:100-200); IF(IHC-P)(IF(IHC-P)) (1:100-200); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does p57 Kip2/Cdkn1c Recombinant Antibody (bsm-61145R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61145R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise p57 Kip2/Cdkn1c Recombinant Antibody (bsm-61145R)?

KLH conjugated synthetic peptide derived from human p57 Kip2

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