p57 Kip2/Cdkn1c Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61145r-cy3-100uL |
| Product Name | p57 Kip2/Cdkn1c Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human p57 Kip2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1028 |
| Swiss Prot | P49918 |
| Subcellular location | Nucleus |
| Synonyms | BWCR; BWS; KIP2; WBS; p57; p57 Kip2; WBS ; CDKN1C; Cyclin dependent kinase inhibitor 1C. |
| Background | Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF(IHC-P) | IFIHC-P1:100-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is p57 Kip2/Cdkn1c Recombinant Antibody, Cy3 Conjugated (bsm-61145R-Cy3) validated for, and at what dilution?
bsm-61145R-Cy3 is validated for WB(WB) (1:1000-2000); IF(IHC-P)(IF(IHC-P)) (1:100-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p57 Kip2/Cdkn1c Recombinant Antibody, Cy3 Conjugated (bsm-61145R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61145R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p57 Kip2/Cdkn1c Recombinant Antibody, Cy3 Conjugated (bsm-61145R-Cy3)?
KLH conjugated synthetic peptide derived from human p57 Kip2
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61145R-Cy3)?
Cy3 on bsm-61145R-Cy3: excitation / emission 512,550nm/570,615nm.