Phospho-AKT1S1 (Thr246) Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61148r-bf647 |
| Product Name | Phospho-AKT1S1 (Thr246) Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AKT1S1 around the phosphorylation site of T246 |
| Modification Site | Thr246 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84335 |
| Swiss Prot | Q96B36 |
| Subcellular location | Cytoplasm |
| Synonyms | AKT1S1; PRAS; PRAS40. |
| Background | Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is Phospho-AKT1S1 (Thr246) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61148R-BF647) validated for, and at what dilution?
bsm-61148R-BF647 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-AKT1S1 (Thr246) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61148R-BF647) react with?
Tested reactivity: Human, Mouse.
How should bsm-61148R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-AKT1S1 (Thr246) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61148R-BF647)?
KLH conjugated synthetic peptide derived from human AKT1S1 around the phosphorylation site of T246
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61148R-BF647)?
AbBy Fluor-647 on bsm-61148R-BF647: excitation / emission 650nm/665nm.