DAPK1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61153r-cy3-100uL |
| Product Name | DAPK1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DAP kinase 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1612 |
| Swiss Prot | P53355 |
| Subcellular location | Cytoplasm |
| Synonyms | DAK1; DAP K1; DAP kinase 1; DAPK 1; DAPK; DAPK1; DAPK1_HUMAN; Death Associated Protein Kinase 1; Death-associated protein kinase 1. |
| Background | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is DAPK1 Recombinant Antibody, Cy3 Conjugated (bsm-61153R-Cy3) validated for, and at what dilution?
bsm-61153R-Cy3 is validated for IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DAPK1 Recombinant Antibody, Cy3 Conjugated (bsm-61153R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61153R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DAPK1 Recombinant Antibody, Cy3 Conjugated (bsm-61153R-Cy3)?
KLH conjugated synthetic peptide derived from human DAP kinase 1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61153R-Cy3)?
Cy3 on bsm-61153R-Cy3: excitation / emission 512,550nm/570,615nm.