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PELP1 Recombinant Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61154r-bf405-100uL
Product Name PELP1 Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PELP1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 27043
Swiss Prot Q8IZL8
Synonyms HMX3; MNAR; P160; PELP1; PELP1 proline glutamic acid leucine rich protein 1; PELP1 proline- glutamic.
Background Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors.Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is PELP1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61154R-BF405) validated for, and at what dilution?

bsm-61154R-BF405 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PELP1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61154R-BF405) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61154R-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PELP1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61154R-BF405)?

KLH conjugated synthetic peptide derived from human PELP1

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-61154R-BF405)?

AbBy Fluor-405 on bsm-61154R-BF405: excitation / emission 401nm/421nm.