ASK1 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61166r-bf488-100uL |
| Product Name | ASK1 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ASK1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4217 |
| Swiss Prot | Q99683 |
| Subcellular location | Cytoplasm |
| Synonyms | Apoptosis signal- regulating kinase 1;ASK-1;MAP3K5;ASK1;MAPKKK5; MEK kinase 5;MEKK5. |
| Background | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is ASK1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61166R-BF488) validated for, and at what dilution?
bsm-61166R-BF488 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ASK1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61166R-BF488) react with?
Tested reactivity: Human, Mouse.
How should bsm-61166R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ASK1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61166R-BF488)?
KLH conjugated synthetic peptide derived from human ASK1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61166R-BF488)?
AbBy Fluor-488 on bsm-61166R-BF488: excitation / emission 499nm/519nm.