LEF1 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61170r-100uL |
| Product Name | LEF1 Recombinant Antibody |
| Applications |
WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)
IP
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LEF1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 51176 |
| Swiss Prot | Q9UJU2 |
| Subcellular location | Nucleus |
| Synonyms | LEF1_HUMAN; Lymphoid enhancer binding factor 1; TCF1 alpha; TCF10; Transcription factor T cell specific 1 alpha; TCF7L3. |
| Background | Transcription factor that binds DNA in a sequence-specific manner.Participates in the Wnt signaling pathway.Activates transcription of target genes in the presence of CTNNB1 and EP300.PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. |
| Application Dilution | |
| WB | 1:1000-2000 |
| FCM | FCM1ug/Test |
| IHC-P | IHC-P1:100-500 |
| IHC-F | IHC-F1:100-500 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
| IP | IP1:20-50 |
Frequently asked questions
Which applications is LEF1 Recombinant Antibody (bsm-61170R) validated for, and at what dilution?
bsm-61170R is validated for WB(WB) (1:1000-2000); FCM(FCM) (1ug/Test); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LEF1 Recombinant Antibody (bsm-61170R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61170R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise LEF1 Recombinant Antibody (bsm-61170R)?
KLH conjugated synthetic peptide derived from human LEF1